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rdf:type |
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lifeskim:mentions |
umls-concept:C0205245,
umls-concept:C0249197,
umls-concept:C0288472,
umls-concept:C0598467,
umls-concept:C1420626,
umls-concept:C1514562,
umls-concept:C1524063,
umls-concept:C1880022,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
2
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pubmed:dateCreated |
1985-9-4
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pubmed:databankReference |
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pubmed:abstractText |
Comparison of the predicted amino acid sequences of different members of the ras family in vertebrates has shown that the N-terminal 120 residues are highly conserved while the C terminus is variable. To test the possible role of the variable residues in cell transformation, chimeras were constructed containing the N-terminal 111 amino acids of the human Ha-ras EJ oncogene and the C terminus of two Drosophila ras genes. We show that one of these constructs which has only 20 conserved residues between positions 121 and 189, can transform rat-1 cells, and the transformed cells are capable of inducing lethal tumors in rats. The second construct containing the C terminus of another Drosophila ras gene exhibits a transforming capacity as well, but only after linkage to a viral transcriptional promoter. These results show that the majority of residues within the C terminus can be replaced without abolishing the transforming potential of p21 ras.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6087162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6147754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6148703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6253666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6253810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6287572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6287573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6290897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6298635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6308465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6308466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6321035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6328429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6330729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6356358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6365329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6430564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6616621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6796966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-6866079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-7133135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3926484-822353
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
407-12
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3926484-Amino Acid Sequence,
pubmed-meshheading:3926484-Animals,
pubmed-meshheading:3926484-Cell Transformation, Neoplastic,
pubmed-meshheading:3926484-Cells, Cultured,
pubmed-meshheading:3926484-DNA,
pubmed-meshheading:3926484-DNA, Recombinant,
pubmed-meshheading:3926484-Drosophila melanogaster,
pubmed-meshheading:3926484-GTP-Binding Proteins,
pubmed-meshheading:3926484-Humans,
pubmed-meshheading:3926484-Neoplasm Proteins,
pubmed-meshheading:3926484-Neoplasms, Experimental,
pubmed-meshheading:3926484-Oncogenes,
pubmed-meshheading:3926484-Proto-Oncogene Proteins p21(ras),
pubmed-meshheading:3926484-Rats,
pubmed-meshheading:3926484-Structure-Activity Relationship
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pubmed:year |
1985
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pubmed:articleTitle |
Characterization of functional domains of p21 ras by use of chimeric genes.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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