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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1985-9-4
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pubmed:abstractText |
Transgenic mice containing a microinjected rearranged immunoglobulin (Ig) mu heavy chain gene were examined for the effects on DNA rearrangement of the endogenous Ig genes. Abelson murine leukemia virus (A-MuLV) cell lines were isolated from pre-B cells of transgenic mice and of normal littermates. Microinjected mu gene RNA and a mu heavy chain protein were synthesized in every transgenic A-MuLV cell line. Only 10% of normal mouse A-MuLV transformants synthesized mu protein. A germ-line JH allele was observed in 40% of the transgenic lines, demonstrating that the block to endogenous Ig DNA rearrangement occurred at the first step of heavy chain DNA joining. All alleles were rearranged in normal mouse A-MuLV lines. Germline JH alleles were also detected in 10% of the transgenic hybridomas derived from proliferating B cells. Our results support a model of active prevention of rearrangement by the product of successfully rearranged mu genes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
117-27
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3926323-Abelson murine leukemia virus,
pubmed-meshheading:3926323-Alleles,
pubmed-meshheading:3926323-Animals,
pubmed-meshheading:3926323-B-Lymphocytes,
pubmed-meshheading:3926323-Cell Differentiation,
pubmed-meshheading:3926323-Cell Line,
pubmed-meshheading:3926323-Cell Transformation, Viral,
pubmed-meshheading:3926323-Immunoglobulin Fragments,
pubmed-meshheading:3926323-Immunoglobulin Heavy Chains,
pubmed-meshheading:3926323-Immunoglobulin J-Chains,
pubmed-meshheading:3926323-Immunoglobulin Light Chains,
pubmed-meshheading:3926323-Immunoglobulin mu-Chains,
pubmed-meshheading:3926323-Mice,
pubmed-meshheading:3926323-Mice, Inbred C57BL,
pubmed-meshheading:3926323-Microinjections,
pubmed-meshheading:3926323-RNA, Messenger,
pubmed-meshheading:3926323-Transcription, Genetic
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pubmed:year |
1985
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pubmed:articleTitle |
A transgenic immunoglobulin mu gene prevents rearrangement of endogenous genes.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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