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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1985-8-14
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pubmed:databankReference |
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pubmed:abstractText |
A cDNA sequence coding for a human granulocyte-macrophage colony-stimulating factor has been isolated from cDNA libraries prepared from mRNA derived from concanavalin A-activated human T-cell clones. The libraries constructed in the pcD vector system were screened by transfecting COS-7 monkey cells with DNA pools to express the products encoded by full-length cDNA inserts. By assaying the cell supernatants, we identified clones encoding a factor that stimulates the formation of granulocyte and macrophage colonies from human progenitor cells. These results demonstrate that identification of full-length cDNAs for many colony-stimulating factors may be achieved entirely on the basis of detecting the functional polypeptide produced in mammalian cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-2856931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-313822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-3918306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-4811820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-6192434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-6216281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-6300662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-6322187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-6345794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-6390681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-6420702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-6610831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-6785749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-6969609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-6973347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-6979717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-7002232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3925454-7443522
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4360-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3925454-Amino Acid Sequence,
pubmed-meshheading:3925454-Animals,
pubmed-meshheading:3925454-Base Sequence,
pubmed-meshheading:3925454-Bone Marrow,
pubmed-meshheading:3925454-Clone Cells,
pubmed-meshheading:3925454-Colony-Stimulating Factors,
pubmed-meshheading:3925454-Concanavalin A,
pubmed-meshheading:3925454-DNA,
pubmed-meshheading:3925454-Gene Expression Regulation,
pubmed-meshheading:3925454-Granulocytes,
pubmed-meshheading:3925454-Haplorhini,
pubmed-meshheading:3925454-Humans,
pubmed-meshheading:3925454-Lymphocyte Activation,
pubmed-meshheading:3925454-Macrophages,
pubmed-meshheading:3925454-Mice,
pubmed-meshheading:3925454-Nucleic Acid Hybridization,
pubmed-meshheading:3925454-T-Lymphocytes,
pubmed-meshheading:3925454-T-Phages,
pubmed-meshheading:3925454-Transfection
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pubmed:year |
1985
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pubmed:articleTitle |
Isolation of cDNA for a human granulocyte-macrophage colony-stimulating factor by functional expression in mammalian cells.
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pubmed:publicationType |
Journal Article,
Comparative Study
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