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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
1985-8-16
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pubmed:abstractText |
We have isolated from an arginase-deficient polyamine-dependent Chinese hamster ovary cell line a new mutant strain that has greatly increased ornithine decarboxylase activity. This enables the cells, in the absence of ornithine, to decarboxylate lysine into cadaverine (diaminopentane) that is further converted into N-(3-aminopropyl)cadaverine and N,N'-bis(3-aminopropyl)cadaverine. These unusual polyamines can support the growth of the cells without added polyamines derived from ornithine. Immunoreactive ornithine decarboxylase-like protein was clearly increased in the mutant cells but could not solely account for the greatly increased enzyme activity. Southern blot analysis of DNA hybridized to a plasmid carrying ornithine decarboxylase-cDNA revealed at least a 32-fold amplification of the ornithine decarboxylase gene. Ornithine decarboxylase-mRNA concentration was also highly increased in the cells. The half-life of the enzyme and the Km for ornithine were not altered from those of the parental cell line.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
260
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8532-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3924915-Adenosylmethionine Decarboxylase,
pubmed-meshheading:3924915-Animals,
pubmed-meshheading:3924915-Cadaverine,
pubmed-meshheading:3924915-Cell Line,
pubmed-meshheading:3924915-Cricetinae,
pubmed-meshheading:3924915-Cricetulus,
pubmed-meshheading:3924915-Eflornithine,
pubmed-meshheading:3924915-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3924915-Female,
pubmed-meshheading:3924915-Gene Amplification,
pubmed-meshheading:3924915-Karyotyping,
pubmed-meshheading:3924915-Kinetics,
pubmed-meshheading:3924915-Lysine,
pubmed-meshheading:3924915-Ornithine,
pubmed-meshheading:3924915-Ornithine Decarboxylase,
pubmed-meshheading:3924915-Ovary,
pubmed-meshheading:3924915-Plasmids,
pubmed-meshheading:3924915-Polyamines,
pubmed-meshheading:3924915-RNA, Messenger,
pubmed-meshheading:3924915-Spermine
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pubmed:year |
1985
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pubmed:articleTitle |
Amplification of ornithine decarboxylase gene in response to polyamine deprivation in Chinese hamster ovary cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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