Linked Life Data

3923934

Source:http://linkedlifedata.com/resource/pubmed/id/3923934

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1985-6-24
pubmed:abstractText
Stimulation of Madin-Darby canine kidney cells by 12-O-tetradecanoyl-phorbol-13-acetate (TPA) results in an increase in prostaglandin synthesis. We have measured the specific activity of the cyclooxygenase system in a cell-free assay and demonstrate that a fourfold induction occurs by 6 h poststimulation. This induction could be prevented by continuous treatment with either cycloheximide or actinomycin D. When cycloheximide was added to cells 6 h post-TPA stimulation we found only a 50% reduction in prostaglandin synthesis over the ensuing 6 h in vitro; this decrease in activity was not observed when actinomycin was used. On the other hand, cells stimulated with TPA for 6 h and subsequently treated with cycloheximide or actinomycin D ceased prostaglandin synthesis completely within 1 h. These results suggest that TPA stimulation of prostaglandin synthesis requires both transcriptional and translational events but that a factor(s) in addition to or in place of the cyclooxygenase might be crucial.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0003-9861
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
239
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
242-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Regulation of arachidonic acid metabolism in Madin-Darby canine kidney cells: stimulation of synthesis of the cyclooxygenase system by 12-O-tetradecanoyl-phorbol-13-acetate.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.