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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1985-7-12
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pubmed:abstractText |
Stereologic techniques were used to analyse the density and distribution of Concanavalin-A (Con-A) receptor sites on the surface of isolated resting human peripheral-blood T lymphocytes using Con-A/colloidal-gold-labelled horseradish peroxidase. The T-lymphocyte surface appeared to be composed of microvilli, smooth areas and uncoated pits. Coated pits and coated vesicles, identified by the preferential staining of clathrin-containing membranes (tannic-acid/saponin fixation), were scarce. Quantitative analysis of the gold labelling on T lymphocytes after glutaraldehyde fixation indicated the presence of 2.13 +/- 0.46 gold particles per micron of cell surface and that these particles were preferentially located on uncoated pits. These results suggest the existence of cell-surface domains for these receptor sites in human resting T lymphocytes.
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pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:issn |
0301-5564
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
82
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
293-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3922926-Coated Pits, Cell-Membrane,
pubmed-meshheading:3922926-Colloids,
pubmed-meshheading:3922926-Gold,
pubmed-meshheading:3922926-Horseradish Peroxidase,
pubmed-meshheading:3922926-Humans,
pubmed-meshheading:3922926-Receptors, Concanavalin A,
pubmed-meshheading:3922926-T-Lymphocytes
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pubmed:year |
1985
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pubmed:articleTitle |
Distribution of concanavalin-A receptor sites on the surface of human resting T lymphocytes. A stereological study using concanavalin-A/colloidal-gold-labelled horseradish peroxidase.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|