Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1985-4-15
|
pubmed:abstractText |
In vitro stimulation of human blood lymphocytes with mitogen resulted in an increased intracellular content of Ca2+ per unit cell volume. This increase in Ca2+ content of lectin-activated cells reached a maximum after 24 hr of culture and thereafter slowly declined. Brief treatment of cells at 24 hr of culture with the Ca2+ ionophore A23187 in combination with EGTA resulted in a larger release of Ca2+ from cells in mitogen-stimulated cultures than from cells in control cultures. This indicates that the Ca2+ is accumulated intracellularly but is readily exchangeable. At 24 hr of culture the increase in cellular Ca2+ correlated well with the proliferative response as measured by 3H-thymidine incorporation. Ca2+ influx at 24 and 48 hr of culture was markedly enhanced in the mitogenically stimulated cells as compared either to cells cultured for 1 and 72 hr or cells cultured without mitogen.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcimycin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0021-9541
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
123
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
46-50
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:3919035-Biological Transport,
pubmed-meshheading:3919035-Calcimycin,
pubmed-meshheading:3919035-Calcium,
pubmed-meshheading:3919035-Cells, Cultured,
pubmed-meshheading:3919035-Concanavalin A,
pubmed-meshheading:3919035-DNA,
pubmed-meshheading:3919035-Egtazic Acid,
pubmed-meshheading:3919035-Humans,
pubmed-meshheading:3919035-Kinetics,
pubmed-meshheading:3919035-Lymphocyte Activation,
pubmed-meshheading:3919035-T-Lymphocytes,
pubmed-meshheading:3919035-Thymidine
|
pubmed:year |
1985
|
pubmed:articleTitle |
Kinetics of long-term (72 hr) calcium content during mitogen activation of cultured human T lymphocytes.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|