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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
|
pubmed:dateCreated |
1985-4-24
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pubmed:abstractText |
Addition of glucagon (20 nM) to the isolated hepatocytes from 24-h starved male rats results in an inactivation of glycogen synthase. The A0.5 for glucose-6-P is increased 2-fold over the control but the S0.5 for UDP-glucose is not significantly affected. The glucagon-stimulated inactivation of glycogen synthase is also accompanied by a 60-120% increase in the phosphorylation of the synthase. Glycogen synthase labeled with 32P by incubation of the hepatocytes with [32P] PO4(3-) was recovered by immunoprecipitation and the resulting immunoprecipitate was subjected to tryptic digestion. Analysis of the 32P-labeled peptides reveals that the sites corresponding to those phosphorylated by cAMP-dependent protein kinase and glycogen synthase (casein) kinase-1 (Itarte, E., and Huang, K.-P. (1979) J. Biol. Chem. 254, 4052-4057) are rapidly phosphorylated in response to glucagon. These results demonstrate that glucagon not only triggers the activation of cAMP-dependent protein kinase through an increase in the intracellular level of cAMP but also, by an unknown mechanism, activates a Ca2+- and cAMP-independent protein kinase.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
25
|
pubmed:volume |
260
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
3239-42
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:3919019-Animals,
pubmed-meshheading:3919019-Chromatography, Thin Layer,
pubmed-meshheading:3919019-Glucagon,
pubmed-meshheading:3919019-Glycogen Synthase,
pubmed-meshheading:3919019-Isoelectric Focusing,
pubmed-meshheading:3919019-Liver,
pubmed-meshheading:3919019-Male,
pubmed-meshheading:3919019-Phosphorylation,
pubmed-meshheading:3919019-Protein Kinases,
pubmed-meshheading:3919019-Rats,
pubmed-meshheading:3919019-Rats, Inbred Strains,
pubmed-meshheading:3919019-Trypsin
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pubmed:year |
1985
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pubmed:articleTitle |
Glucagon-stimulated phosphorylation of rat liver glycogen synthase in isolated hepatocytes.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|