Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-3-1
pubmed:abstractText
Phosphorylation of ribosomal protein S6 in NIH 3T3 fibroblasts is dependent on the presence of serum, but after transformation of these cells by Abelson murine leukemia virus (Ab-MuLV), S6 remained highly phosphorylated on serine residues either in the absence or the presence of serum. To investigate whether S6 phosphorylation in this system was a consequence of the action of the Ab-MuLV tyrosine-specific protein kinase, purified Ab-MuLV kinase made in Escherichia coli was microinjected into Xenopus oocytes and was observed to cause a 7- to 15-fold increase in the phosphorylation of S6 on serine residues. Two-dimensional phosphopeptide maps of S6 phosphorylated in Ab-MuLV-transformed NIH cells in the absence of serum were identical to those of S6 isolated from normal cells grown in the presence of serum. In addition, S6 from oocytes injected with Ab-MuLV kinase yielded an S6 phosphopeptide map indistinguishable from that of serum-stimulated NIH 3T3 cells, whereas S6 from control oocytes lacked several phosphopeptides. Ab-MuLV kinase did not phosphorylate S6 directly in vitro, and microinjection of a mutant Ab-MuLV protein lacking kinase activity had no effect. These results indicate that the Ab-MuLV kinase interacts with a cellular pathway to enhance S6 phosphorylation by directly or indirectly activating an S6 protein kinase and/or inactivating an S6 protein phosphatase.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-190233, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-4423396, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-511842, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-571354, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6093101, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6157683, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6173766, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6177870, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6178977, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6179940, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6193890, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6194425, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6232463, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6244493, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6246443, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6246487, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6255480, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6257396, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6258311, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6267586, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6269932, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6270659, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6279626, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6292177, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6302192, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6309774, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6312321, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6313661, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6319014, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6319390, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6323411, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6325456, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6350281, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6365656, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6402364, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6417656, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6436688, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6571834, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6601328, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6643464, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6645975, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6751557, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6759164, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6804946, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-6852247, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-7045876, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-7075598, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918307-7317068
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
272-6
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1985
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