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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1980-3-17
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pubmed:abstractText |
A novel approach for the analysis of membrane proteins involved in ligand-induced surface receptor patching and capping is described. The technique is based on the use of immunolactoperoxidase (immuno-LPO) conjugates which catalyze the iodination of those surface proteins with available tyrosine groups that are located in the immediate vicinity of the patch or cap of a particular antigen. We have used the patching and capping of the H-2 (histocompatibility) antigen on mouse thymocytes to illustrate this method. However, this technique should be generally applicable to any cell surface proteins which can be induced to form patches or caps by a specific ligand. Cytochemical analysis indicates that the immuno-LPO conjugates induce the same patching and capping of the H-2 antigen as does the unconjugated antibody. Biochemical analysis of the 125I-labeled proteins by SDS polyacrylamide gel electrophoresis indicates that a large membrane protein (mol wt of approximately 200,000 daltons) is closely associated with H-2 patches and caps. Since a number of other prominent membrane proteins are not labeled by this procedure, selective redistribution of certain surface proteins must be occurring during H-2 antibody-induced patching and capping.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-1082455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-1102608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-163833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-307764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-4119593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-4119937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-4140515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-4333397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-4544022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-4569171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-4594420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-4597237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-4611172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-46243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-4626851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-47173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-5127429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-5354774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-54922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-61878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/391811-7179
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
649-56
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:391811-Animals,
pubmed-meshheading:391811-Antibodies,
pubmed-meshheading:391811-Antigen-Antibody Complex,
pubmed-meshheading:391811-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:391811-H-2 Antigens,
pubmed-meshheading:391811-Immunoenzyme Techniques,
pubmed-meshheading:391811-Immunologic Capping,
pubmed-meshheading:391811-Membrane Proteins,
pubmed-meshheading:391811-Mice,
pubmed-meshheading:391811-Receptors, Antigen, T-Cell,
pubmed-meshheading:391811-T-Lymphocytes
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pubmed:year |
1979
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pubmed:articleTitle |
Biochemical analysis of ligand-induced receptor patching and capping using a novel immunolactoperoxidase iodination technique.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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