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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1985-3-20
|
| pubmed:abstractText |
The phenotype of B lineage cells (TdT+, pre-B, IgM+, IgD-, and IgM+,IgD+) in infant and adult human bone marrow was compared with that of B cells seen in peripheral tissues such as tonsil and blood. The range of B cell-associated antibodies used included four reagents with greater than 90% reactivity on peripheral B cells: RFB4 and To15 (both p135, corresponding to CD22), RFB6 (p140 corresponding to CD21), and Y29/55, a unique B cell-specific antibody. In addition, AL-1, an antibody with virtually no reactivity against peripheral B cells was also used. The BM cell subpopulations were heterogeneous in respect of antibody reactivity. The TdT+, pre-B and IgM+, IgD- cells were AL-1+ but did not express membrane antigens recognized by the antibodies To15, RFB4 (CD22), and RFB6 (CD21). TdT+, pre-B cells, and 50% of IgM+, IgD- BM B cells were also unreactive with antibody Y29/55, the other 50% being Y29/55+. In contrast, the IgM+,IgD+ BM B cells, like peripheral B cells, were positive with antibodies To15, RFB4, RFB6, and Y29/55, but reacted only in small numbers with AL-1. The orderly differentiation-linked display of these antigens was also suggested by the findings that normal TdT+, pre-B, and IgM+,IgD- cells expressed the To15 and RFB4 (CD22) antigens in their cytoplasm (in the Golgi region). This observation was confirmed in malignant common acute lymphoblastic and pre-B blast cells, as well as in the corresponding permanent cell lines KM3 and NALM-6. In these lines the membrane expression of To15 and RFB4 could be induced by phorbol ester during a 48 to 72 hr culture period.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
134
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1524-30
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:3918103-Adolescent,
pubmed-meshheading:3918103-Adult,
pubmed-meshheading:3918103-Animals,
pubmed-meshheading:3918103-Antibodies, Monoclonal,
pubmed-meshheading:3918103-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:3918103-Antigens, Surface,
pubmed-meshheading:3918103-B-Lymphocytes,
pubmed-meshheading:3918103-Bone Marrow,
pubmed-meshheading:3918103-Bone Marrow Cells,
pubmed-meshheading:3918103-Cell Line,
pubmed-meshheading:3918103-Child,
pubmed-meshheading:3918103-Child, Preschool,
pubmed-meshheading:3918103-Cytoplasm,
pubmed-meshheading:3918103-Goats,
pubmed-meshheading:3918103-Humans,
pubmed-meshheading:3918103-Leukemia, Lymphoid,
pubmed-meshheading:3918103-Lymphoid Tissue,
pubmed-meshheading:3918103-Palatine Tonsil,
pubmed-meshheading:3918103-Regeneration
|
| pubmed:year |
1985
|
| pubmed:articleTitle |
Human B cell development. I. Phenotypic differences of B lymphocytes in the bone marrow and peripheral lymphoid tissue.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|