Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-3-20
pubmed:databankReference
pubmed:abstractText
A 2.3-kilobase pair EcoRI fragment containing the spo0B locus has been sequenced. The spo0B locus was shown to code for a protein of 22,542 daltons. Promoter distal to the spo0B locus, an open reading frame was uncovered which was preceded by a strong ribosome-binding site. S1 nuclease protection experiments revealed that both the spo0B locus and this open reading frame were part of the same transcript. A portion of the middle of the open reading frame was cloned in the integrative vector pJH101. Transformation of this plasmid into Bacillus subtilis 168 was only rarely successful, and those few colonies that arose consisted of cells that had lost the plasmid. The results suggested that the product of this open reading frame is essential for the growth of the bacterium. The regulation of the spo0B locus was studied by using translational spo0B-lacZ fusions in an integrative vector. These studies revealed that the spo0B locus was maximally expressed during vegetative growth. It was estimated that 50 to 100 copies of the protein are present during this period. Sequence analysis of the region upstream from the spo0B locus revealed another operon that contained a gene coding for a protein homologous to ribosomal protein L27 of Escherichia coli.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-1225626, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-16561900, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-2981817, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-383576, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-387076, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-388356, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-4199504, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-4956288, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-6162838, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-6246368, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-6260747, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-6260752, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-6269464, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-6269757, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-6290454, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-6296629, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-6304019, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-6313226, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-6322121, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-6402773, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-6415045, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-6790515, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-821319, http://linkedlifedata.com/resource/pubmed/commentcorrection/3918016-922889
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
161
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
556-62
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Sequence analysis of the spo0B locus reveals a polycistronic transcription unit.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.