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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1985-3-20
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pubmed:abstractText |
The nucleoid of living and OsO4- or glutaraldehyde-fixed cells of Escherichia coli strains was studied with a phase-contrast microscope, a confocal scanning light microscope, and an electron microscope. The trustworthiness of the images obtained with the confocal scanning light microscope was investigated by comparison with phase-contrast micrographs and reconstructions based on serially sectioned material of DNA-containing and DNA-less cells. This comparison showed higher resolution of the confocal scanning light microscope as compared with the phase-contrast microscope, and agreement with results obtained with the electron microscope. The effects of fixation on the structure of the nucleoid were studied in E. coli B/r H266. Confocal scanning light micrographs and electron microscopic reconstructions showed that the shape of the nucleoid remained similar after OsO4 or glutaraldehyde fixation; however, the OsO4 nucleoid appeared to be somewhat smaller and more centralized within the cell.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-1107308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-13319263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-13610928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-13976593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-13986422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-4556329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-4580561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-4866336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-5327372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-5337775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-6389508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-6767695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-6784179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-7045374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-7075699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-821931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3918013-94144
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
161
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
478-83
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1985
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pubmed:articleTitle |
Confocal scanning light microscopy of the Escherichia coli nucleoid: comparison with phase-contrast and electron microscope images.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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