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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1 Pt 2
|
| pubmed:dateCreated |
1985-1-14
|
| pubmed:abstractText |
In frog fundic mucosae mounted in Ussing chambers, exposure to luminal 1 M NaCl for 10 min caused a sharp immediate decrease in potential difference, resistance, short circuit current, and acid secretion, but within 4-6 h these readings had returned toward control values. After initial severe destruction of surface epithelial cells, gradual morphologic restitution occurred within 4-6 h. A Ca2+-free nutrient solution and 4 mM ethylenediaminetetraacetic acid administered after injury prevented both physiologic and morphologic restitution. A Ca2+-free nutrient solution administered alone after injury prevented physiologic recovery, but although narrow gaps and lack of tight junctions were found between some cells, there was near-complete epithelial cell coverage. The addition of 2 mM Ca2+ to these tissues 3 h after injury effected rapid recovery of electrophysiologic parameters and a complete closure of the intercellular spaces. Cytochalasin B (3 X 10(-3) M nutrient) prevented physiologic recovery and mucosal restitution. Neither cycloheximide nor colchicine had any effect on the normal process of restitution. Autoradiography of [3H]thymidine incorporation showed no increase in labeling within 4 h of hyperosmolar injury. We conclude that adequate Ca2+ is required for complete restitution of gastric mucosa after hyperosmolar injury, and that restitution occurs by migration of persisting viable gastric pit cells.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Colchicine,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochalasin B,
http://linkedlifedata.com/resource/pubmed/chemical/Edetic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Leucine,
http://linkedlifedata.com/resource/pubmed/chemical/Metiamide,
http://linkedlifedata.com/resource/pubmed/chemical/Saline Solution, Hypertonic,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0016-5085
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
88
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
237-49
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:3917262-Animals,
pubmed-meshheading:3917262-Autoradiography,
pubmed-meshheading:3917262-Calcium,
pubmed-meshheading:3917262-Colchicine,
pubmed-meshheading:3917262-Cycloheximide,
pubmed-meshheading:3917262-Cytochalasin B,
pubmed-meshheading:3917262-Cytoskeleton,
pubmed-meshheading:3917262-Edetic Acid,
pubmed-meshheading:3917262-Epithelium,
pubmed-meshheading:3917262-Gastric Juice,
pubmed-meshheading:3917262-Gastric Mucosa,
pubmed-meshheading:3917262-Intercellular Junctions,
pubmed-meshheading:3917262-Leucine,
pubmed-meshheading:3917262-Metiamide,
pubmed-meshheading:3917262-Microtubules,
pubmed-meshheading:3917262-Protein Biosynthesis,
pubmed-meshheading:3917262-Rana catesbeiana,
pubmed-meshheading:3917262-Regeneration,
pubmed-meshheading:3917262-Saline Solution, Hypertonic,
pubmed-meshheading:3917262-Thymidine
|
| pubmed:year |
1985
|
| pubmed:articleTitle |
Requirements for restitution of the surface epithelium of frog stomach after mucosal injury.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
|