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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1986-3-31
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pubmed:abstractText |
Heat-stable enterotoxin (STa) isolated from bovine Escherichia coli strains was purified to homogeneity by growing the bacterial strains in a chemically defined medium, desalting, and concentrating the culture filtrate by batch adsorption chromatography on Amberlite XAD-2 resin, batch adsorption chromatography on reversed-phase silica, and preparative reversed-phase high-performance liquid chromatography. This rapid preparative purification scheme gave high recovery yields of pure STa which exhibited biochemical homology to STa purified by more complicated procedures.
|
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
|
pubmed:issn |
0003-2697
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
151
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
431-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3913332-Amino Acids,
pubmed-meshheading:3913332-Animals,
pubmed-meshheading:3913332-Bacterial Toxins,
pubmed-meshheading:3913332-Biological Assay,
pubmed-meshheading:3913332-Cattle,
pubmed-meshheading:3913332-Chromatography, Gel,
pubmed-meshheading:3913332-Chromatography, High Pressure Liquid,
pubmed-meshheading:3913332-Chromatography, Ion Exchange,
pubmed-meshheading:3913332-Enterotoxins,
pubmed-meshheading:3913332-Escherichia coli,
pubmed-meshheading:3913332-Mice,
pubmed-meshheading:3913332-Species Specificity
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pubmed:year |
1985
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pubmed:articleTitle |
Preparative purification of Escherichia coli heat-stable enterotoxin.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|