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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1986-3-31
|
| pubmed:abstractText |
An improved enzymatic method for the determination of pyrroloquinoline quinone, a novel prosthetic group of some important oxidoreductases, has been developed with cytoplasmic membrane of Escherichia coli K-12, in which D-glucose dehydrogenase (EC 1.1.99.17) was completely resolved to apo-enzyme by EDTA treatment. Incubation of the EDTA-treated membrane with exogenous pyrroloquinoline quinone in the presence of magnesium ions gave a quantitative determination of pyrroloquinoline quinone by assaying the restored D-glucose dehydrogenase activity. This novel enzymatic method was confirmed to be highly reproducible up to 10 ng of pyrroloquinoline quinone and could be applied to a routine assay of pyrroloquinoline quinone.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0003-2697
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
151
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
263-7
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3913327-Cell Membrane,
pubmed-meshheading:3913327-Coenzymes,
pubmed-meshheading:3913327-Escherichia coli,
pubmed-meshheading:3913327-Glucose Dehydrogenases,
pubmed-meshheading:3913327-Hydrogen-Ion Concentration,
pubmed-meshheading:3913327-Kinetics,
pubmed-meshheading:3913327-PQQ Cofactor,
pubmed-meshheading:3913327-Quinolines
|
| pubmed:year |
1985
|
| pubmed:articleTitle |
Method of enzymatic determination of pyrroloquinoline quinone.
|
| pubmed:publicationType |
Journal Article
|