Linked Life Data

3909852

Source:http://linkedlifedata.com/resource/pubmed/id/3909852

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1986-2-17
pubmed:abstractText
Addition of 0.5 microgram/ml of the red fluorescent dye propidium iodide to the penultimate wash of Xenopus early embryo serial sections during immunofluorescence studies causes germ plasm to fluoresce brightly. Germ plasm can be accurately and speedily identified in the serial sections. The use of this dye is a marked improvement over previous methods of germ plasm identification in unstained sections. Studies involving screening for germ-plasm-specific antibodies are greatly facilitated by staining germ plasm red with propidium iodide and searching for green colabelling by fluoresceinated antibodies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0003-276X
pubmed:author
pubmed:issnType
Print
pubmed:volume
213
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
518-9, 536-7
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Use of the fluorochrome propidium iodide for the identification of Xenopus germ plasm during immunofluorescence studies.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.