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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1986-2-7
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pubmed:abstractText |
A simple procedure for the preparation of 10-500 mg of the Escherichia coli elongation Tu-Ts complex is described. The protocol is based on the separate purification and quantitation of EF-Tu-GDP and EF-Ts, followed by mixing of equimolar amounts of each protein and removal of the displaced GDP by dialysis. Single crystals grown from the final product have been analyzed by X-ray diffraction techniques. The procedure is also applicable to the bulk preparation and crystallization of the trypsin-modified Tu-Ts complex. Quantitation of the elongation factors by three methods is presented.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0003-2697
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
150
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
243-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3909845-Chromatography, Gel,
pubmed-meshheading:3909845-Crystallization,
pubmed-meshheading:3909845-Dialysis,
pubmed-meshheading:3909845-Escherichia coli,
pubmed-meshheading:3909845-Guanosine Diphosphate,
pubmed-meshheading:3909845-Peptide Elongation Factor Tu,
pubmed-meshheading:3909845-Peptide Elongation Factors,
pubmed-meshheading:3909845-Protein Binding,
pubmed-meshheading:3909845-Trypsin,
pubmed-meshheading:3909845-X-Ray Diffraction
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pubmed:year |
1985
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pubmed:articleTitle |
Bulk preparation and crystallization of the Escherichia coli elongation factor Tu-Ts complex.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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