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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1986-1-30
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pubmed:abstractText |
The technical complexity of determining the serovar of Chlamydia trachomatis strains has limited the use of serotyping in clinical and epidemiologic studies. We developed a simple method for rapidly serotyping isolates of C. trachomatis by using monoclonal antibodies in a dot-enzyme-linked immunosorbent assay (ELISA) system. Isolates were passaged three to six times in shell vial cultures to greater than 50% monolayer infection, and chlamydial elementary bodies were isolated by sonication and microcentrifugation. Chlamydial antigen was spotted onto a series of replicate nitrocellulose membrane patches and reacted with C. trachomatis-specific monoclonal antibodies. Bound antibody was detected visually by a color reaction by using peroxidase-conjugated anti-mouse immunoglobulins. This method can be routinely applied to 60 or more specimens concurrently. We compared dot-ELISA serotyping with monoclonal antibody microimmunofluorescence serotyping of 124 clinical C. trachomatis isolates and found that dot-ELISA has sensitivity and serotyping accuracy comparable to that of monoclonal antibody microimmunofluorescence.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-1172191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-13798522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-14050195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-16558075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-340953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-4186430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-4210884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-4631875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-4855645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-6067314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-6179965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-622087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-6323579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-6874078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-6955786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-7017409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-7019235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-7035557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-7072935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-810514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-831128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3908472-859544
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
609-13
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:3908472-Antibodies, Bacterial,
pubmed-meshheading:3908472-Antibodies, Monoclonal,
pubmed-meshheading:3908472-Antigens, Bacterial,
pubmed-meshheading:3908472-Chlamydia Infections,
pubmed-meshheading:3908472-Chlamydia trachomatis,
pubmed-meshheading:3908472-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:3908472-Fluorescent Antibody Technique,
pubmed-meshheading:3908472-Humans,
pubmed-meshheading:3908472-Serotyping
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pubmed:year |
1985
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pubmed:articleTitle |
Rapid immunotyping of Chlamydia trachomatis with monoclonal antibodies in a solid-phase enzyme immunoassay.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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