Linked Life Data

3908411

Source:http://linkedlifedata.com/resource/pubmed/id/3908411

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1986-2-19
pubmed:abstractText
Using immunocytochemical techniques, we report here direct evidence of kynurenine (Kyn) presence and localization in the lung and brain. Kyn is a metabolite of tryptophan and 5-hydroxytryptophan, produced by indoleamine 2,3-dioxygenase (IDO). Whereas IDO has been quantitated in tissues from lung, brain, and other organs, Kyn has only been identified in brain (by HPLC), and its specific localization has not been determined. We reacted alternate serial paraffin sections with antisera raised in rabbits against a L-Kyn-albumin conjugate, and with anti-5HT (serotonin, 5-hydroxytryptamine), using the PAP method. Kyn-like immunoreactivity in the lung was specifically localized to cells of the bronchiolar epithelium resembling basal cells. Taller epithelial cells in the bronchi and dorsal trachea were likewise positive whereas neuroepithelial bodies were negative. Immunoreactivity in the brain was typically localized to cells localized in the ependyma of the walls of all ventricles, and to nerve fibers. The cellular Kyn-like reactivity was totally separate from that of anti-5HT, the latter uniquely staining argyrophil lung neuroendocrine cells and raphae neurons of the brain. Our findings suggest a route of tryptophan metabolism in the lung and brain alternate to the common pathway leading to 5-hydroxyindoleacetic acid via 5-HT. This route is of physiologic and pathologic significance as many metabolites are pharmacologically active.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0301-5564
pubmed:author
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
465-71
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Evidence for presence of kynurenine in lung and brain of neonate hamsters.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't