| pubmed-article:3907624 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3907624 | lifeskim:mentions | umls-concept:C0040300 | lld:lifeskim |
| pubmed-article:3907624 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:3907624 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:3907624 | lifeskim:mentions | umls-concept:C0040711 | lld:lifeskim |
| pubmed-article:3907624 | lifeskim:mentions | umls-concept:C0076726 | lld:lifeskim |
| pubmed-article:3907624 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:3907624 | lifeskim:mentions | umls-concept:C1514468 | lld:lifeskim |
| pubmed-article:3907624 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:3907624 | pubmed:dateCreated | 1986-1-21 | lld:pubmed |
| pubmed-article:3907624 | pubmed:abstractText | A panel of six mouse monoclonal antibodies (IgG1) has been prepared against purified rat urinary kallikrein (EC 3.4.21.35) and characterized. In radioimmunoassay, the antibody titres of ascitic fluid giving 50% binding to 125I-kallikrein range from 1:2 X 10(3) to 1:1 X 10(6). Antibodies from four of the clones show no cross-reactivity with human urinary kallikrein, rat urinary esterase A or tonin. However, antibodies from a fifth clone cross-react with tonin and, from a sixth, with both urinary esterase A and tonin. Three of the kallikrein affinity-purified monoclonal antibodies inhibited, whereas one of the antibodies stimulated, kallikrein activity. Tissue kallikrein from rat submandibular-gland and pancreatic extracts and urine were labelled with [14C]di-isopropyl phosphofluoridate, immunoprecipitated with each of the six monoclonal antibodies and identified to be 38 kDa proteins, similar in size to purified rat urinary kallikrein. Western-blot analysis shows that 125I-labelled kallikrein monoclonal antibodies (V4D11) bind directly to a 38 kDa protein in submandibular-gland and pancreatic extracts and urine. Cell-free translation products of submandibular-gland polyadenylylated[poly(A)+]mRNA were immunoprecipitated with affinity-purified sheep anti-kallikrein antibodies and three monoclonal antibodies (V4D11, V4G6 and V1C3). Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of these immunoprecipitates revealed that two kallikrein precursors with Mr values of 37 000 and 35 000 are encoded by submandibular-gland mRNA. The third monoclonal antibody, V1C3, which binds to active kallikrein, did not recognize either precursor form. Collectively, the data show that these monoclonal antibodies comprise a set of powerful and specific reagents for studies of tissue kallikreins. | lld:pubmed |
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| pubmed-article:3907624 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:3907624 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3907624 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3907624 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:3907624 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:3907624 | pubmed:author | pubmed-author:MargoliusH... | lld:pubmed |
| pubmed-article:3907624 | pubmed:author | pubmed-author:ChaoJJ | lld:pubmed |
| pubmed-article:3907624 | pubmed:author | pubmed-author:ChanMM | lld:pubmed |
| pubmed-article:3907624 | pubmed:author | pubmed-author:WoodleyC MCM | lld:pubmed |
| pubmed-article:3907624 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3907624 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:3907624 | pubmed:volume | 231 | lld:pubmed |
| pubmed-article:3907624 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3907624 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3907624 | pubmed:pagination | 721-8 | lld:pubmed |
| pubmed-article:3907624 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:3907624 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:3907624 | pubmed:articleTitle | Specific identification of tissue kallikrein in exocrine tissues and in cell-free translation products with monoclonal antibodies. | lld:pubmed |
| pubmed-article:3907624 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3907624 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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