Linked Life Data

3905003

Source:http://linkedlifedata.com/resource/pubmed/id/3905003

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1986-1-23
pubmed:abstractText
An immunohistochemical study was carried out on a human breast carcinoma series with the aim to delineate the technical approach to distinguish the epithelial cells from stromal nonepithelial cells in the primary tumor and in cultured tumor cells. Frozen sections from 30 unfixed breast carcinomas were incubated with mouse monoclonal antiepithelial cell membrane antibodies and then with a biotinilizated antimouse antibody and the avidin biotin peroxidase complex (ABC). Thick (100 micron) sections from fresh, unfixed tissue were similarly treated prior to the araldite embedding procedure for EM study. In all cases the epithelial tumor cells were immunostained in contrast to nonepithelial stromal cells, particularly the fibroblasts. Because standard light microscopy fails to determine the real nature of the fusiforme cultured cells, it is suggested that these techniques represent a reliable method for mammary epithelial cell identification and may further be applied on cultured tissue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0361-090X
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
77-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Immunocytochemical antigens detection in human breast carcinomas: a light and electron microscopy study using avidin biotin peroxidase and preembedding method.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't