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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1985-12-6
|
| pubmed:abstractText |
beta-Lactamase from Bacillus licheniformis 749/c was used as a marker for ELISA. Conjugates of the beta-lactamase with the capsule antigen of the plague causative agent and with the monoclonal antibodies to it were prepared by glutaric aldehyde "linking". The conjugates preserved high immunospecific and beta-lactamase activity. High sensitivity of the modification of ELISA allowed detecting up to 8 ng/ml of the capsule antigen of the plague causative agent. The enzymatic activity of the beta-lactamase was determined microiodometrically which provided visual recording of the results of ELISA.
|
| pubmed:language |
rus
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0233-7525
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
30
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
662-5
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3904622-Antibodies, Monoclonal,
pubmed-meshheading:3904622-Antigens, Bacterial,
pubmed-meshheading:3904622-Bacillus,
pubmed-meshheading:3904622-Immunoenzyme Techniques,
pubmed-meshheading:3904622-Indicators and Reagents,
pubmed-meshheading:3904622-Yersinia pestis,
pubmed-meshheading:3904622-beta-Lactamases
|
| pubmed:year |
1985
|
| pubmed:articleTitle |
[Use of beta-lactamase from Bacillus licheniformis 749/C for solid-phase immunoenzyme analysis].
|
| pubmed:publicationType |
Journal Article,
English Abstract
|