Linked Life Data

3903495

Source:http://linkedlifedata.com/resource/pubmed/id/3903495

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1985-12-20
pubmed:abstractText
Phosphorylating mitochondrial membranes were obtained from Trypanosoma cruzi culture (epimastigote) forms. Using ADP as phosphate acceptor and succinate, sn-glycerol-3-phosphate, L-malate or ascorbate plus tetramethyl-p-phenylenediamine (TMPD) as oxidizable substrates, energy coupling sites II and III were detected, with respiratory control values in the range of 2.8-2.0. Carbonyl cyanide m-chlorophenylhydrazone and sonication uncoupled the respiratory control mechanism. Antimycin and cyanide partially inhibited succinate, sn-glycerol-3-phosphate and L-malate oxidation, while cyanide totally inhibited ascorbate + TMPD oxidation by the mitochondrial preparation. Succinate oxidation was inhibited by malonate and oxalacetate, but this latter was only effective with sonicated mitochondria. At variance with other substrates, NADH oxidation was not controlled by ADP concentration or inhibited by antimycin or cyanide. Rotenone failed to inhibit electron transfer in T. cruzi mitochondria.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0166-6851
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
289-98
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Respiratory control in mitochondria from Trypanosoma cruzi.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't