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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1985-12-10
|
| pubmed:abstractText |
The use of a mixed pH indicator containing bromocresol purple and bromothymol blue was evaluated for the detection of beta-lactamase activity in enzyme immunoassays (EIA) based on this enzyme. The EIA was found to correlate well with a bioassay for antibodies to tetanus toxoid in sheep sera. Results could be read spectrophotometrically at 450 nm or visually as a colour change from dark blue to yellow. The mixed pH indicator was found to have a number of desirable features including good stability, ease of preparation and a sharp colour change which makes the system suitable for visual determination of titration end points.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0022-1759
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
7
|
| pubmed:volume |
83
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
371-7
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:3902981-Animals,
pubmed-meshheading:3902981-Antibodies, Bacterial,
pubmed-meshheading:3902981-Bromcresol Purple,
pubmed-meshheading:3902981-Bromthymol Blue,
pubmed-meshheading:3902981-Clostridium tetani,
pubmed-meshheading:3902981-Hydrogen-Ion Concentration,
pubmed-meshheading:3902981-Immunoenzyme Techniques,
pubmed-meshheading:3902981-Sheep,
pubmed-meshheading:3902981-Spectrophotometry,
pubmed-meshheading:3902981-beta-Lactamases
|
| pubmed:year |
1985
|
| pubmed:articleTitle |
An evaluation of the use of a pH indicator for the detection of beta-lactamase in enzyme immunoassay.
|
| pubmed:publicationType |
Journal Article
|