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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1985-11-4
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pubmed:abstractText |
A cDNA clone for the 90kDa heat-shock protein, which we have recently identified as a component of steroid hormone receptors in their heteromeric 8S form, was isolated by direct immunological screening of a chicken smooth muscle cDNA expression library, prepared in the expression plasmids pUC8 and pUC9. Using polyclonal and monoclonal antibodies against the 90kDa protein a colony was identified that reacted with both antibodies. Plasmid 9.11 (p9.11, approximately 1100 base pair insert) was found to hybrid-select mRNA for the 90kDa heat-shock protein. Northern blot analysis revealed that RNA isolated from various chicken tissues contain a single transcript of approximately 3 Kb hybridizing to a [32P]labelled cDNA probe made from p9.11. Heat-shock treatment of chick embryonic fibroblasts resulted in increased steady-state levels of a 3 Kb transcript in both poly A+ and poly A- RNA fractions. Southern blot analysis of chicken genomic DNA indicated that the cDNA hybridizes to a single copy sequence. Sequence data show that the p9.11 cDNA displays a high degree of homology with the 5' portion of yeast heat shock protein 90 cDNA.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-1055375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-1105573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-117907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-351332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-3965298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6118854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6166005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6185958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6201134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6201744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6207179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6262754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6296817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6298609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6300896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6310323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6314271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6325446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6403517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6493229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6539778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6546970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6548747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6574454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6772444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6780199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-6997135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-7110134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-7140763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-823012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-890741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3900927-911786
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6035-47
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3900927-Amino Acid Sequence,
pubmed-meshheading:3900927-Animals,
pubmed-meshheading:3900927-Base Composition,
pubmed-meshheading:3900927-Base Sequence,
pubmed-meshheading:3900927-Chickens,
pubmed-meshheading:3900927-Cloning, Molecular,
pubmed-meshheading:3900927-DNA,
pubmed-meshheading:3900927-Genetic Vectors,
pubmed-meshheading:3900927-Heat-Shock Proteins,
pubmed-meshheading:3900927-Nucleic Acid Hybridization,
pubmed-meshheading:3900927-Protein Biosynthesis,
pubmed-meshheading:3900927-RNA, Messenger,
pubmed-meshheading:3900927-Rabbits,
pubmed-meshheading:3900927-Reticulocytes,
pubmed-meshheading:3900927-Saccharomyces cerevisiae,
pubmed-meshheading:3900927-Species Specificity
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pubmed:year |
1985
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pubmed:articleTitle |
Cloning of the chick hsp 90 cDNA in expression vector.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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