Linked Life Data

3899044

Source:http://linkedlifedata.com/resource/pubmed/id/3899044

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1985-10-16
pubmed:abstractText
DNA from the marine bacterium Alteromonas haloplanktis 214 was partially digested with Sau 3A and inserted into the Bam HI site of the cloning vector pBR322. The ligation mixture was used to transform Escherichia coli HB101. The gene bank plasmid preparation obtained was used to transform Escherichia coli K-12 strain EO2717, an organism auxotrophic for histidine, arginine, adenine, uracil and thiamin. Prototrophic transformants for each of the five metabolites were isolated using appropriate minimal media for selection. Plasmids isolated from each of the transformants were shown by hybridization to contain A. haloplanktis DNA and to be capable of complementing the appropriate mutation in E. coli EO2717. Restriction maps showed that each of the plasmids was different.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0302-8933
pubmed:author
pubmed:issnType
Print
pubmed:volume
142
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
248-52
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Expression of genes from the marine bacterium Alteromonas haloplanktis 214 in Escherichia coli K-12.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't