Linked Life Data

3896523

Source:http://linkedlifedata.com/resource/pubmed/id/3896523

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-10-4
pubmed:abstractText
A rabbit antibody against a chemically synthesized peptide (p84), encompassing residues 59-72 of mature human interleukin 2 (IL-2), has been shown to react specifically with natural or recombinant IL-2. This antibody was used in immunoperoxidase and immunofluorescence techniques for identification of IL-2-containing cells in human peripheral blood or tonsils. Lymphocytes were stimulated with T-cell mitogens (PHA, PWM), fixed, and incubated with affinity-purified anti-p84 antibody, followed by appropriately conjugated secondary antibodies. FACS analysis demonstrated a low fluorescence intensity in 5 to 15% of unstimulated cells. In contrast, 40-60% of mitogen-stimulated cells were stained at a high fluorescence intensity. Staining was inhibited by preincubating the anti-p84 antibody with the homologous peptide or recombinant IL-2, but not by unrelated peptides. In immunoperoxidase staining, anti-p84 antibody reacted selectively with an enriched T-cell population which was 95% Leu 5+, 80% Leu 3+, and 60% Tac+. Thus, this antibody to a synthetic IL-2 peptide reacts selectively with activated T cells, and may serve, therefore, as a useful tool for visualization and enumeration of IL-2-containing cells in blood and tissues.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0008-8749
pubmed:author
pubmed:issnType
Print
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
491-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Staining and fluorescence-activated cell sorter analysis of human lymphocytes using antibodies to a short, chemically synthesized human IL-2 peptide.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't