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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0019735,
umls-concept:C0022827,
umls-concept:C0024348,
umls-concept:C0033684,
umls-concept:C0039195,
umls-concept:C0086418,
umls-concept:C0221284,
umls-concept:C0456387,
umls-concept:C0524637,
umls-concept:C0591833,
umls-concept:C1171362,
umls-concept:C1420192,
umls-concept:C1514873,
umls-concept:C1515670,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C1704222
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pubmed:issue |
13
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pubmed:dateCreated |
1985-8-14
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pubmed:abstractText |
To dissect the molecular basis for T-cell recognition of class I major histocompatibility complex antigens, we have examined the ability of human cytotoxic T lymphocytes (CTL) to recognize murine L cells transformed with a human class I gene. Three transformed L-cell lines were generated that expressed the human HLA-A3 gene from donor E1 at levels comparable to those of the endogenous L-cell H-2Kk molecules. CTL were generated in secondary and tertiary mixed lymphocyte culture against the HLA-A3 subtype of donor E1 by culturing irradiated E1 peripheral blood lymphocytes with the peripheral blood lymphocytes of responder donor M3 (M3 shares all defined class I antigens with E1 but expresses a different HLA-A3 subtype). Each of the HLA-A3-transformed L cells was lysed by M3 anti-E1 CTL in a short-term 51Cr release assay and this recognition was blocked by a monoclonal anti-HLA-A3 antibody. Antibodies specific for the human T8 and LFA-1 molecules on the CTL effectors (but absent from the transformed targets) also blocked lysis of each of the HLA-A3 transformed L-cell targets. Antibodies to other T-cell molecules failed to block lysis. The present results demonstrate that human CTL can recognize human class I molecules on targets that do not express any other human gene product and further suggest that effector T-cell molecules T8 and LFA-1 are functionally involved in this recognition process.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-157372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-314668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-3871112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-3880579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-4018029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-48197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-567555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6153199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6165083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6184620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6185618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6191218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6191921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6198392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6199334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6199452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6201533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6208124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6208306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6234599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6286765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6333726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6339253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6345670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6352810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6369147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6390442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6436376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6436377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6609814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6610558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6815536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6975312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6977452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-6978378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-92183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3892536-95015
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4490-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3892536-Humans,
pubmed-meshheading:3892536-Animals,
pubmed-meshheading:3892536-Mice,
pubmed-meshheading:3892536-Proteins,
pubmed-meshheading:3892536-Fibroblasts,
pubmed-meshheading:3892536-Fluorescent Antibody Technique,
pubmed-meshheading:3892536-Transformation, Genetic,
pubmed-meshheading:3892536-Aspartate Carbamoyltransferase,
pubmed-meshheading:3892536-HLA Antigens,
pubmed-meshheading:3892536-Gene Expression Regulation,
pubmed-meshheading:3892536-Multienzyme Complexes,
pubmed-meshheading:3892536-Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing),
pubmed-meshheading:3892536-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:3892536-Antibodies, Monoclonal,
pubmed-meshheading:3892536-Dihydroorotase,
pubmed-meshheading:3892536-HLA-A3 Antigen
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