rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
4
|
pubmed:dateCreated |
1985-7-31
|
pubmed:abstractText |
Incubation of photomixotrophic cell suspension cultures of rape (Brassica napus) and heterotrophic cell suspension cultures of soya (Glycine max) with 1-O-[1'-14C]hexadecyl-sn-glycerol or rac-1-O-[1'-14C]hexadecylglycerol leads in high yield (up to 78%) to labeled 1-O-hexadecyl-2-acyl-sn-glycero-3-phosphocholines. Alkaline hydrolysis of the choline glycerophospholipids yields pure 1-O-[1'-14C]hexadecyl-sn-glycero-3-phosphocholine. 1-O-[1'-14C]Hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor) is obtained by acetylating the lyso compound. The semi-synthetic preparation described leads to labeled platelet activating factor in an overall yield of 50-60% without loss of specific activity.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0022-2275
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
26
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
495-500
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:3891896-Brassica,
pubmed-meshheading:3891896-Carbon Radioisotopes,
pubmed-meshheading:3891896-Cells, Cultured,
pubmed-meshheading:3891896-Indicators and Reagents,
pubmed-meshheading:3891896-Palmitic Acid,
pubmed-meshheading:3891896-Palmitic Acids,
pubmed-meshheading:3891896-Plants,
pubmed-meshheading:3891896-Platelet Activating Factor,
pubmed-meshheading:3891896-Radioisotope Dilution Technique,
pubmed-meshheading:3891896-Soybeans
|
pubmed:year |
1985
|
pubmed:articleTitle |
Semi-synthetic preparation of 1-O-[1'-14C]hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor) using plant cell cultures.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|