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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1985-8-5
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pubmed:abstractText |
We immunocytochemically stained rat pituitary glands using antibodies against plasminogen activators of the tissue type (t-PA) and the urokinase type (u-PA). A large population of endocrine cells in the anterior lobe of the gland displayed intense cytoplasmic immunoreactivity with anti-t-PA. In some areas of the intermediate lobe we found a weak staining, and we observed weakly staining granular structures in the posterior lobe. Controls included absorption of the antibodies with highly purified t-PA. In addition, SDS PAGE followed by immunoblotting of pituitary gland extracts revealed only one band with an electrophoretic mobility similar to that of t-PA when stained with anti-t-PA IgG. No u-PA immunoreactivity was detected in the rat pituitary gland. Sequential staining experiments using antibodies against growth hormone and t-PA demonstrated that the t-PA-immunoreactive cells constitute a large subpopulation of the growth hormone-containing cells. These findings represent the first direct evidence for the presence of t-PA in cell types other than endothelial cells in the intact normal organism. In this article we discuss the implications of the results for a possible role of t-PA in the posttranslational processing of prohormones.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-11894897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-13444042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-151588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-207771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-214238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-4226004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-4347290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-455456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6089198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-61067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6166029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6248125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6256003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6275379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6284507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6311839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6337343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6365928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6418388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6444726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6450468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6538482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6538515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6572897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6750605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6754572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6806270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6808517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6873305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6891264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-6891463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-7041809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-7191151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-78958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3891762-975245
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
101
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
305-11
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1985
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pubmed:articleTitle |
Immunocytochemical demonstration of tissue-type plasminogen activator in endocrine cells of the rat pituitary gland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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