Linked Life Data

3888261

Source:http://linkedlifedata.com/resource/pubmed/id/3888261

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1985-7-2
pubmed:abstractText
The fluorescence of the aliphatic (amino acido)-N2-methyl-9-hydroxyellipticinium (AA-NMHE) derivatives [Auclair, C., Voisin, E., Banoun, H., Bernardou, J., Meunier, B., & Paoletti, C. (1984) J. Med. Chem. 27, 1161-1166], namely, dehydroglycino-NMHE, dehydroalanino-NMHE, dehydrovalino-NMHE, and dehydroleucino-NMHE, has been characterized. The changes in the fluorescence properties of the drugs, including increase in quantum yields, increase in fluorescence lifetimes, and occurrence of energy transfer upon binding to DNA in vitro, have been further investigated. The measurement of the fluorescence increment of AA-NMHE when bound to fluorescent sites inside intact bacteria has been found to be suitable for the determination of the accessibility of the drugs to bacterial nucleic acids according to the method of Lambert and Le Pecq [Lambert, B., & Le Pecq, J.B. (1984) Biochemistry 23, 166-176]. With this methodology, the kinetics of drug uptake, the ability of the drug to reach the bacterial nucleic acids at equilibrium, and the nature of the ligand binding model have been determined in two AA-NMHE-sensitive strains, Escherichia coli BL 101 (Lambert & Le Pecq, 1984) and Salmonella typhimurium TA 98 [Ames, B.N., Lee, F.D., & Durston, W.E. (1973) Proc. Natl. Acad. Sci. U.S.A. 70, 782-786]. The main results obtained are the following: At nonsaturating concentrations, each AA-NMHE exhibits a marked difference in its ability to reach the bacterial nucleic acids. This parameter seems to be correlated with the antibacterial efficiency of the drugs.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
701-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Accessibility to bacterial nucleic acids of the intercalating drug aliphatic amino acid ellipticinium derivatives in Escherichia coli and Salmonella typhimurium.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't