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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1985-6-3
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pubmed:abstractText |
The bulk of an open reading frame extending from map coordinates 23.3 to 14.2 in region E2b of the adenoviral genome has been cloned and expressed from a chimeric plasmid in Escherichia coli. The cloning strategy used created a fusion protein of 124,000 daltons, which contained greater than 98% adenovirus-encoded sequences. Antiserum raised against this protein reacted with the authentic 140,000-dalton adenovirus DNA polymerase. Another serum raised against a synthetic hexapeptide whose sequence corresponded to the predicted carboxyl terminus of adenovirus-encoded DNA polymerase also reacted with the fusion protein and authentic adenovirus DNA polymerase. These results demonstrate that the cloned region of DNA encodes the adenovirus DNA polymerase.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-195391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-196758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-230480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-368040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-4321915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-487438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6205505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6216480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6258160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6266278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6297162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6297165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6297776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6302192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6308611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6310323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6330082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6330083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6336326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6343626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6374895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6572922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6573663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6722875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6940154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6947251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-6957861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-7086968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-7142161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-7142162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-7142163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-7241655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-7471210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3887401-949741
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2354-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1985
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pubmed:articleTitle |
Expression in Escherichia coli of a fusion protein product containing a region of the adenovirus DNA polymerase.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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