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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1985-5-3
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pubmed:abstractText |
We have purified from Moloney murine leukemia virus (Mo-MuLV) a protease that has the capacity of accurately cleaving the polyprotein precursor Pr65gag into the mature viral structural proteins. Both the NH2- and COOH-terminal amino acid sequences have been determined and aligned with the amino acid sequence deduced from the DNA sequence of Mo-MuLV by other workers. The results show that: (i) the protease is located at the 5' end of the pol gene, and the first four amino acids are overlapped with the 3' end of the gag gene; (ii) the fifth amino acid residue is glutamine, which is inserted by suppression of the UAG termination codon at the gag-pol junction; and (iii) the protease is composed of 125 amino acids with calculated Mr = 13,315, and the COOH terminus of the protease is adjacent to the NH2 terminus of reverse transcriptase. The map order of the gag-pol gene is proposed to be 5'-p15-p12-p30-p10-protease-reverse transcriptase-endonuclease-3'.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-13269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-191840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-202399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-212597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-4331554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-5661631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6160262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6169994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6175245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6178211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6197537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6205170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6268146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6299578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6304725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6308662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6319746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6322438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6328019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6333515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-634374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6408267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6577423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-67705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6912798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-6964389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-72613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-7356143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3885215-78989
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1618-22
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1985
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pubmed:articleTitle |
Murine leukemia virus protease is encoded by the gag-pol gene and is synthesized through suppression of an amber termination codon.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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