Linked Life Data

3884179

Source:http://linkedlifedata.com/resource/pubmed/id/3884179

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1985-4-26
pubmed:abstractText
The reverse haemolytic plaque assay was developed to measure antigen (AgE or rye grass I)-specific IgE-plaque-forming cells from the peripheral-blood lymphocytes of ragweed- and grass-allergic patients. The anti-IgE-developing antisera was shown to be isotype-specific, and the response of the assay was inhibited by 52% by the addition of 10 pg of antigen. In addition, the assay was shown to have a reproducibility (s.d.) of 15%. The blood lymphocytes from all fifteen atopic (grass and ragweed) patients were shown to form antigen-specific IgE-plaque-forming cells during the pollen season (mean value 115 cells) and up to 6 months after the season (mean value 56 cells). Cycloheximide appears to block the formation of the plaque-forming cells. This method appears to be sensitive and reproducible enough to study in vitro IgE antibody synthesis of peripheral-blood lymphocytes from atopics.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0009-9090
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-53
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Detection of antigen-specific IgE-plaque-forming cells from peripheral-blood lymphocytes of ragweed- and grass-allergic patients.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro