Linked Life Data

3882834

Source:http://linkedlifedata.com/resource/pubmed/id/3882834

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-3-26
pubmed:abstractText
A reliable source of positive control material is needed for immunoperoxidase or immunofluorescent terminal deoxynucleotidyl transferase (TdT) assays. The cell line RPMI 8402 was obtained from a patient with acute lymphoblastic leukemia and is positive for nuclear TdT. The kinetics of TdT expression in a series of cultured RPMI 8402 cells and the effect of cryopreservation on TdT expression were evaluated. The highest percentage of TdT+ cells was detected on days 5-8 of RPMI 8402 cell cultures. TdT positivity was preserved for more than 3 years in cells stored in suspension at -70 degrees C, and at least 76 days on slide preparations when fixed with methanol prior to freezing. We conclude that when RPMI 8402 cells with maximum TdT positivity are stored at -70 degrees C, aliquots can be used as a constant source of positive control material thereby eliminating the necessity and expense of a continuing culture system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
205-9
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Frozen RPMI 8402 cells: a reliable source of terminal deoxynucleotidyl transferase positive control material.
pubmed:publicationType
Journal Article