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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1985-4-18
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pubmed:abstractText |
DNA obtained from Chlamydia trachomatis (serovar L2) was partially digested with DNase I and inserted into the beta-galactosidase gene of bacteriophage lambda gt11. Seven recombinants were selected that produced immunoreactive fusion proteins which were detected with anti-C. trachomatis rabbit serum. One recombinant, designated lambda gt11/L2/33, reacted with various monoclonal antibodies that recognize species-, subspecies-, and type-specific determinants on the chlamydial major outer membrane protein (MOMP). Immunoblot analysis of a lambda gt11/L2/33 lysogen revealed a fusion protein that expressed a approximately 15,000-dalton carboxyl-terminal peptide of the chlamydial MOMP. This moiety of the MOMP possesses epitopes responsible for each of the unique reactivities demonstrated by anti-MOMP monoclonal antibodies. The lambda gt11/L2/33 recombinant contained a 1.1-kilobase DNA insert which hybridized to DNA isolated from each of the 15 C. trachomatis serovars.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-1097546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-1376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-16557794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-4915925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-6184717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-6185424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-6219389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-6266279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-7002026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-7035557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-7068209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-7152681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-7217005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3882565-7228399
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
713-8
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:3882565-Antibodies, Monoclonal,
pubmed-meshheading:3882565-Antigens, Bacterial,
pubmed-meshheading:3882565-Bacterial Outer Membrane Proteins,
pubmed-meshheading:3882565-Chlamydia trachomatis,
pubmed-meshheading:3882565-Cloning, Molecular,
pubmed-meshheading:3882565-DNA, Bacterial,
pubmed-meshheading:3882565-Escherichia coli,
pubmed-meshheading:3882565-Molecular Weight,
pubmed-meshheading:3882565-Species Specificity
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pubmed:year |
1985
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pubmed:articleTitle |
Molecular cloning and expression of Chlamydia trachomatis major outer membrane protein antigens in Escherichia coli.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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