Linked Life Data

3882421

Source:http://linkedlifedata.com/resource/pubmed/id/3882421

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1985-4-10
pubmed:abstractText
The transcriptional rates of four different genes in shoots of barley grown under different light regimes were quantified by monitoring nuclear RNA transcripts using gene-specific hybridization probes. Isolated nuclei were pulse-labelled with [alpha-32P]UTP and the relative rates of light-harvesting chlorophyll a/b protein (LHCP) mRNA, NADPH:protochlorophyllide oxidoreductase mRNA, B1 hordein mRNA, and 26-S rRNA synthesis were measured. Irradiation of dark-grown plants with a red light pulse increased the rate of LHCP mRNA synthesis tenfold within 3 h, and the rate of rRNA synthesis more than twofold within 9 h. The relative rate of synthesis of the oxidoreductase mRNA decreased following a red light pulse reaching a minimum after 3-6 h. As a direct proof of phytochrome involvement in the light-induced stimulation of LHCP and the repression of the oxidoreductase transcripts for both responses, red/far-red reversibility could be demonstrated. We conclude that phytochrome is able both to increase the transcription of certain nuclear genes and decrease the transcription of others.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
147
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-42
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed-meshheading:3882421-Cell Nucleus, pubmed-meshheading:3882421-Cereals, pubmed-meshheading:3882421-Chlorophyll, pubmed-meshheading:3882421-DNA, pubmed-meshheading:3882421-Gene Expression Regulation, pubmed-meshheading:3882421-Hordeum, pubmed-meshheading:3882421-Light, pubmed-meshheading:3882421-Light-Harvesting Protein Complexes, pubmed-meshheading:3882421-Nucleic Acid Hybridization, pubmed-meshheading:3882421-Oxidation-Reduction, pubmed-meshheading:3882421-Oxidoreductases, pubmed-meshheading:3882421-Oxidoreductases Acting on CH-CH Group Donors, pubmed-meshheading:3882421-Photosynthetic Reaction Center Complex Proteins, pubmed-meshheading:3882421-Phytochrome, pubmed-meshheading:3882421-Plant Proteins, pubmed-meshheading:3882421-RNA, Messenger, pubmed-meshheading:3882421-Transcription, Genetic
pubmed:year
1985
pubmed:articleTitle
Phytochrome control of in vitro transcription of specific genes in isolated nuclei from barley (Hordeum vulgare).
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't