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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1985-4-23
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pubmed:abstractText |
A new technique using immunofluorescence and autoradiography is described, in which the DNA of cells in S phase are labeled with two different probes. This method makes it possible to study the relationship between DNA synthesis and the uptake and/or incorporation of chemotherapeutic agents into normal or neoplastic cells. An example is provided in which the incorporation of 3H-cytarabine into DNA is demonstrated to occur only in cells which were synthesizing DNA during exposure to 3H-cytarabine. Other radioactively labeled probes can be used as well.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0361-5960
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
195-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3882228-Antibodies, Monoclonal,
pubmed-meshheading:3882228-Autoradiography,
pubmed-meshheading:3882228-Bone Marrow,
pubmed-meshheading:3882228-Bromodeoxyuridine,
pubmed-meshheading:3882228-Cells, Cultured,
pubmed-meshheading:3882228-Cytarabine,
pubmed-meshheading:3882228-DNA,
pubmed-meshheading:3882228-Fluorescent Antibody Technique,
pubmed-meshheading:3882228-Humans,
pubmed-meshheading:3882228-Leukemia,
pubmed-meshheading:3882228-Thymidine,
pubmed-meshheading:3882228-Tritium,
pubmed-meshheading:3882228-Tumor Stem Cell Assay
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pubmed:year |
1985
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pubmed:articleTitle |
Double labeling of human leukemic cells using 3H-cytarabine and monoclonal antibody against bromodeoxyuridine.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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