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pubmed:abstractText |
Parechymal cells (PC) were prepared by digestion of perfused, lavaged rat lung in a mixture of collagenase and DNase, and harvested on a discontinuous percoll gradient. The process yielded on average 1.0 X 10(8) viable cells/gram tissue. PC were pulsed with soluble antigen, and tested for their capacity to trigger antigen-specific activation of immune T-cells in vitro, or to replace adherent accessory cells necessary for Concanavalin A (Con A)-induced T-cell proliferation. Unfractionated PC exhibited only minor antigen-presenting cell (APC) activity. However, removal of adherent or FcR-positive cells unmasked substantial APC activity. Subsequent experiments indicated that the majority of the APC banded at the top of the percoll gradient (density less than 1.048 g/ml). The same cell preparations substituted for adherent accessory cells in Con A- activation of T cells, suggesting capacity to secrete soluble factors such as interleukin-1 (IL-1) as well to present antigen. The PC preparation also contained T-cells, which were refractory to Con A stimulation unless endogenous adherent cells were first removed. Collectively, these data suggest the presence of non-adherent, FcR-negative, low density accessory cells in the lung parenchyma, capable of both APC activity and soluble factor production. Their T-cell-activation functions appear to be down regulated by endogenous adherent, FcR-positive cells. It is speculated that the accessory cells in these lung preparations may be dendritic cells, the activity of which is subject to inhibition by macrophages.
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