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rdf:type |
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|
lifeskim:mentions |
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pubmed:dateCreated |
1985-11-12
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|
pubmed:abstractText |
We have used a mammalian cell expression cloning system to identify cDNA clones encoding human granulocyte-macrophage colony stimulating factor. The human clone was used as a hybridization probe to identify the corresponding sequence from a cDNA library prepared from a gibbon T-cell line. The human cDNA has been used to produce recombinant GM-CSF in monkey COS-1 cells. The purified protein from COS cells is very similar to the GM-CSF isolated from a continuous human T-cell line.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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|
pubmed:issn |
0361-7742
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
191
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
351-66
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3876571-Amino Acid Sequence,
pubmed-meshheading:3876571-Animals,
pubmed-meshheading:3876571-Bone Marrow Cells,
pubmed-meshheading:3876571-Cell Line,
pubmed-meshheading:3876571-Colony-Stimulating Factors,
pubmed-meshheading:3876571-DNA,
pubmed-meshheading:3876571-Gene Expression Regulation,
pubmed-meshheading:3876571-Granulocytes,
pubmed-meshheading:3876571-Humans,
pubmed-meshheading:3876571-Interleukin-2,
pubmed-meshheading:3876571-Macrophages,
pubmed-meshheading:3876571-Transfection
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|
pubmed:year |
1985
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|
pubmed:articleTitle |
Isolation of cDNAs encoding human and gibbon GM-CSF.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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