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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1985-10-30
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pubmed:abstractText |
By panning procedures employing T6 and T3 monoclonal antibody, human thymocytes were fractionated into two subpopulations depleted of T6- or T3-positive (T6+, T3+) cells. Unfractionated thymocytes and T6- and T3-depleted subpopulations were separately cultured for 48 h in RPMI 1640 medium with 10% FCS or in HB 101 serum-free medium. Determining the phenotype of unfractionated thymocytes at various time intervals, a time-dependent increase of T3+ cells was observed. An inverse relationship was found between the percentage of T3+ cells and the T6 and peanut agglutinin (PNA) reactive thymocytes. When the surface antigen expression in the T3-depleted population (greater than 95% T6+ and PNA+ cells) was analysed, a strong increase of T3+ cells and a complementary reduction of T6+ and PNA+ cells was evidenced. During that time the surface phenotype of the T6-depleted population (greater than 80% T3+ cells) showed the same trend of differentiation, as the other thymocyte preparations. These results indicate that a conspicuous fraction of human thymocytes and particularly of those characterized by a cortical phenotype (PNA+ and T6+ cells), are able to express mature T-cell antigens when cultured in vitro in the absence of the thymic microenvironment influence. However, the in vitro acquisition of a mature phenotype is not accompanied by a parallel achievement of the capacity to respond to mitogens such as PHA or T3 monoclonal antibody.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3876184-311473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3876184-351618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3876184-3917863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3876184-4539848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3876184-5549896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3876184-6154738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3876184-6191218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3876184-6231110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3876184-6796273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3876184-6965396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3876184-6991122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3876184-71328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3876184-915290
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
433-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3876184-Antibodies, Monoclonal,
pubmed-meshheading:3876184-Antigens, Surface,
pubmed-meshheading:3876184-Cells, Cultured,
pubmed-meshheading:3876184-Child,
pubmed-meshheading:3876184-Child, Preschool,
pubmed-meshheading:3876184-Humans,
pubmed-meshheading:3876184-Interleukin-1,
pubmed-meshheading:3876184-Lectins,
pubmed-meshheading:3876184-Mitosis,
pubmed-meshheading:3876184-Peanut Agglutinin,
pubmed-meshheading:3876184-Phenotype,
pubmed-meshheading:3876184-Phytohemagglutinins,
pubmed-meshheading:3876184-T-Lymphocytes,
pubmed-meshheading:3876184-Time Factors
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pubmed:year |
1985
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pubmed:articleTitle |
T3 expression by human thymocytes in culture.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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