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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1985-10-24
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pubmed:databankReference |
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pubmed:abstractText |
The molecular cloning of bone gamma-carboxyglutamic acid (Gla) protein (BGP; osteocalcin) was accomplished by constructing a phage lambda gt11 cDNA library from the rat osteosarcoma cell line ROS 17/2 and screening this library with antibodies raised against BGP from rat bone. By sequencing several cloned cDNAs, we have established a 489-base-pair sequence that predicts a mature BGP of 50 amino acid residues with an NH2-terminal extension of 49 residues. The leader peptide consists of a hydrophobic signal peptide followed by a basic propeptide of 26 or 27 residues that is cleaved after an Arg-Arg dipeptide prior to secretion from the cell. Mature rat BGP is extremely homologous to BGPs from other species except for its COOH-terminal sequence. A stretch of 9 residues proximal to the NH2 terminus of secreted BGP is strikingly similar to the corresponding regions in known propeptides of the gamma-carboxyglutamic acid-containing blood coagulation factors. We suggest that this common structural feature may be involved in the posttranslational targeting of these polypeptides for vitamin K-dependent gamma-carboxylation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-1059086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-322279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-368799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-3871773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-426799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-524275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-593900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6087294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6091100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6096099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6153795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6171778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6219389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6245680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6295879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6310323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6326805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6330671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6356359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6379599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6423828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-643613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6602126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6687940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6965675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6966406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6967067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6967872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6968673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6969260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6971290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6972763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6975778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6978733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6984192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-6996572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-7014242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-7280687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-822353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-881736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3875856-96437
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6109-13
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3875856-Amino Acid Sequence,
pubmed-meshheading:3875856-Animals,
pubmed-meshheading:3875856-Base Sequence,
pubmed-meshheading:3875856-Calcium-Binding Proteins,
pubmed-meshheading:3875856-Cloning, Molecular,
pubmed-meshheading:3875856-DNA,
pubmed-meshheading:3875856-Osteocalcin,
pubmed-meshheading:3875856-Protein Precursors,
pubmed-meshheading:3875856-Protein Processing, Post-Translational,
pubmed-meshheading:3875856-RNA, Messenger,
pubmed-meshheading:3875856-Rats,
pubmed-meshheading:3875856-Vitamin K
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pubmed:year |
1985
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pubmed:articleTitle |
The propeptide of rat bone gamma-carboxyglutamic acid protein shares homology with other vitamin K-dependent protein precursors.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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