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rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
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pubmed:dateCreated |
1985-9-16
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|
pubmed:abstractText |
A derivative of human blood clotting factor IXa beta lacking gamma-carboxyglutamic acid (Gla) residues was prepared by limited proteolysis with chymotrypsin, and subsequently examined for its ability to bind calcium ions. By amino acid analysis, Gla-domainless human factor IXa beta contained 0.3-0.4 moles of beta-hydroxyaspartic acid per mole of protein. Equilibrium dialysis experiments demonstrated that Gla-domainless human factor IXa beta retained two high-affinity calcium binding sites (Kd=52 microM), a finding essentially identical to that observed for Gla-domainless bovine factor IX that contains 0.8-0.9 moles of beta-hydroxyaspartic acid per mole of protein. These data strongly suggest that the beta-hydroxyaspartic acid residue in these proteins does not participate in their high affinity calcium sites.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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|
pubmed:month |
Jul
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|
pubmed:issn |
0006-291X
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
31
|
|
pubmed:volume |
130
|
|
pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
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|
pubmed:pagination |
841-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3875344-1-Carboxyglutamic Acid,
pubmed-meshheading:3875344-Amino Acids,
pubmed-meshheading:3875344-Aspartic Acid,
pubmed-meshheading:3875344-Binding Sites,
pubmed-meshheading:3875344-Calcium,
pubmed-meshheading:3875344-Chromatography, Ion Exchange,
pubmed-meshheading:3875344-Factor IX,
pubmed-meshheading:3875344-Factor IXa,
pubmed-meshheading:3875344-Glutamates,
pubmed-meshheading:3875344-Humans,
pubmed-meshheading:3875344-Time Factors
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pubmed:year |
1985
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|
pubmed:articleTitle |
Calcium binding to a human factor IXa derivative lacking gamma-carboxyglutamic acid: evidence for two high-affinity sites that do not involve beta-hydroxyaspartic acid.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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