Linked Life Data

3874158

Source:http://linkedlifedata.com/resource/pubmed/id/3874158

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1985-8-16
pubmed:abstractText
A monoclonal antibody to the Ly-6.2 specificity, defined by strain and tissue distribution, was used to identify the cellular antigens of lymphocytes and tumor cell lines. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of immune precipitates demonstrated that the Ly-6.2 antigen on the surface of thymus and the T lymphoma EL-4 was a protein of 34 kd, whereas spleen cells showed two Ly-6.2 molecules of 34 kd and 56 kd. In vitro translation of EL-4 T-lymphoma poly A+ RNA followed by immunoprecipitation showed the synthesis of two Ly-6.2 precursor polypeptides. These two precursors were translated from separable mRNA molecules; the larger encoded a 54 kd polypeptide, while the second, smaller one translated a 36 kd polypeptide. Thus, the T lymphoma EL-4 contains two distinct mRNA, but only one is seen on the surface, while spleen cells contain and translate both mRNAs and both surface forms are detected. What determines the utilization of the two mRNAs and the surface expression of the two different proteins in the different tissues is not known. Whether the two mRNAs are the transcripts of one gene or arise by transcription of two separate but closely linked genes remains to be determined.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0093-7711
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
539-47
pubmed:dateRevised
2010-10-5
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Identification of two forms of the Ly-6.2 antigen showing differential expression.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't