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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1985-7-24
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pubmed:abstractText |
Cytochrome P-450-dependent aryl hydrocarbon hydroxylase (AHH) and 7-ethoxycoumarin O-deethylase activities of a cloned line of human lymphoblastoid AHH-1 cells are inhibited by a monoclonal antibody (MAb 1-7-1) prepared to a 3-methylcholanthrene-induced rat liver cytochrome P-450. The monoclonal antibody inhibition determined that a single MAb 1-7-1-sensitive type of cytochrome P-450 is responsible for all of AHH expression in both the basal and benz[a]anthracene-induced cells. Partial inhibition by the MAb 1-7-1, however, indicates that at least two forms of cytochrome P-450 catalyze 7-ethoxycoumarin O-deethylase in both the basal and the induced cells, one form of which is identical to the MAb-sensitive cytochrome P-450 responsible for all of the AHH. Thus, a single cloned cell line is capable of expressing two classes of cytochromes P-450, and the observed multiplicity of cytochrome P-450 in animal tissues does not necessarily depend on cell heterogeneity. A sensitive MAb 1-7-1-based radioimmunoassay also directly demonstrates the presence in these cells of a MAb 1-7-1-specific type of cytochrome P-450 as well as its elevation in the induced cells. These MAb-based methods thus can determine the contribution of specific MAb-defined types of cytochromes P-450 to the cellular metabolism of specific xenobiotics.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0026-895X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
652-5
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:3873598-7-Alkoxycoumarin O-Dealkylase,
pubmed-meshheading:3873598-Animals,
pubmed-meshheading:3873598-Antibodies, Monoclonal,
pubmed-meshheading:3873598-Antigen-Antibody Complex,
pubmed-meshheading:3873598-Aryl Hydrocarbon Hydroxylases,
pubmed-meshheading:3873598-Benz(a)Anthracenes,
pubmed-meshheading:3873598-Cell Line,
pubmed-meshheading:3873598-Clone Cells,
pubmed-meshheading:3873598-Cytochrome P-450 Enzyme System,
pubmed-meshheading:3873598-Humans,
pubmed-meshheading:3873598-Kinetics,
pubmed-meshheading:3873598-Lymphocytes,
pubmed-meshheading:3873598-Methylcholanthrene,
pubmed-meshheading:3873598-Microsomes, Liver,
pubmed-meshheading:3873598-Oxygenases,
pubmed-meshheading:3873598-Radioimmunoassay,
pubmed-meshheading:3873598-Rats
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pubmed:year |
1985
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pubmed:articleTitle |
Monoclonal antibody-directed determination of cytochrome P-450 types expressed in a human lymphoblastoid cell line.
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pubmed:publicationType |
Journal Article
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