rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
1
|
pubmed:dateCreated |
1985-4-12
|
pubmed:abstractText |
A method is described for the purification of sheep lymphocytes carrying class II MHC antigens. After incubation of purified blood lymphocytes on anti-IgM-coated petri dishes, the adherent fraction contained 95% sIg-positive cells determined by immunofluorescence. When tested with cross-reacting anti-class II (bovine and human) monoclonal antibodies, more than 95% of these cells were positive either by immunofluorescence or cytotoxicity. This technique will permit studies of the polymorphism of sheep class II antigens.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0022-1759
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
28
|
pubmed:volume |
77
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
37-43
|
pubmed:dateRevised |
2005-11-17
|
pubmed:meshHeading |
pubmed-meshheading:3871823-Animals,
pubmed-meshheading:3871823-Antibodies, Monoclonal,
pubmed-meshheading:3871823-B-Lymphocytes,
pubmed-meshheading:3871823-Cell Separation,
pubmed-meshheading:3871823-Goats,
pubmed-meshheading:3871823-Histocompatibility Antigens,
pubmed-meshheading:3871823-Leukocytes,
pubmed-meshheading:3871823-Major Histocompatibility Complex,
pubmed-meshheading:3871823-Receptors, Antigen, B-Cell,
pubmed-meshheading:3871823-Sheep,
pubmed-meshheading:3871823-Species Specificity,
pubmed-meshheading:3871823-Swine
|
pubmed:year |
1985
|
pubmed:articleTitle |
A method of purifying sheep sIg+ lymphocytes as a tool for class II MHC antigen analysis.
|
pubmed:publicationType |
Journal Article
|