Linked Life Data

3865240

Source:http://linkedlifedata.com/resource/pubmed/id/3865240

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1986-1-2
pubmed:abstractText
A method for the routine assay of plasma thromboxane and prostacyclin metabolites, thromboxane B2 and 6 keto-prostaglandin F1 alpha (employing solid phase extraction to remove the metabolites from interfering substances) and using commercially available antisera for their radioimmunoassay has been investigated. Modifications of previously published procedures have been explored, including the use of non-explosive solvents in the extraction procedure, and inclusion of a non-cross reacting internal standard, prostaglandin D2, to estimate recovery through the extraction procedures. Both the original and modified procedures can produce high blank values, which apparently result from the solid-phase extraction. These high backgrounds are constant for a given lot of solid phase extraction cartridges and can be corrected by subtraction. The modified method is linear with volume of plasma assayed to as little as one ml, and all cross-reacting material in normal human plasma was found to co-chromatograph with authentic standards on reverse-phase high performance liquid chromatography. Values for normal adult plasmas were found to be 0.44 +/- .15 pmol/ml 6 keto PGF1 alpha (mean +/- SD) and .103 +/- .07 for thromboxane B2. The method reported provides a convenient, reproducible way to assay these important plasma prostanoids.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0262-1746
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
151-67
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Re-examination of the assay for plasma prostanoids by solid-phase extraction, and radioimmunoassay.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't