Linked Life Data

3862114

Source:http://linkedlifedata.com/resource/pubmed/id/3862114

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
1985-10-7
pubmed:abstractText
Epithelial cells of the gallbladder of Necturus maculosus were loaded with tetramethylammonium (Me4N+) by transient exposure of the apical (lumen-facing) surface to a solution of high Me4N+ concentration containing also the polyene antibiotic nystatin. Upon removal of nystatin, in the continued presence of Me4N+, spontaneous restoration of the native ionic permeability of the apical cell membrane was observed. At this time, external Me4N+ was removed; intracellular [Me4N+] measured with ion-sensitive microelectrodes was 2-15 mM and remained unchanged for several hours. Changes in cell volume were estimated from the changes in intracellular [Me4N+] produced by alterations in the osmolality of the mucosal bathing solution. Assuming that such changes are caused entirely by water fluxes across the apical membrane, the minimum value of its hydraulic permeability coefficient (Lp) was 1-3 X 10(-3) cm.sec-1.(osmoles/kg)-1, suggesting that an osmolality difference across the apical membrane as small as 1-3 milliosmoles/kg could explain the average rate of transepithelial water transport. These results agree with optical measurements [Persson, B. O. & Spring, K. R. (1982) J. Gen. Physiol. 79, 481-505]. The effective thickness of the apical unstirred layer was estimated from the time courses of both the apical membrane voltage and the response of an extracellular K+-sensitive microelectrode to an increase in [K+] in the mucosal bath. Since changes in concentration of the osmotically active solute at the membrane surface were thus shown to be significantly delayed by diffusion, the Lp value, calculated assuming a step-change in osmolality, is an underestimate.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-1214280, http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-1214283, http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-1214284, http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-395308, http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-438773, http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-4689624, http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-5084117, http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-5945255, http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-6377340, http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-6481335, http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-6601915, http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-7077291, http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-7077647, http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-7119733, http://linkedlifedata.com/resource/pubmed/commentcorrection/3862114-915470
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6014-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Changes in cell volume measured with an electrophysiologic technique.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.