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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1985-6-25
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pubmed:databankReference |
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pubmed:abstractText |
Two HLA class II beta-chain clones from a cell line homozygous for the DR1 haplotype have been characterized and sequenced. They represent a DR beta chain (2918.4) and a DQ beta chain (2918.8). Clone 2918.4 has been used to select mRNA from a lymphoblastoid cell line, and this was injected into Xenopus oocytes with mRNA selected with a DR alpha chain. The translation products were immunoprecipitated with a beta-chain-specific monoclonal antibody and electrophoresed on two-dimensional gels. This revealed positive signals in the positions predicted for beta and alpha chains. Sequence comparisons of 2918.4 with previously published DR beta-chain sequences confirm the presence of two regions of variability in the membrane distal domain. Analysis of the sequence of 2918.8 identified it as a DQ beta chain identical to one previously published from a DR3,w6 cell line. We speculate, therefore, that the DQ beta sequence represents the DQ1 specificity shared by the DR1 and DRw6 haplotypes.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-11894954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-6136966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-6198089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-6206493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-6324181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-6403940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-6403947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-6585297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-6589154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-6600932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-6947956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-6952222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3858829-6954511
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3405-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3858829-Animals,
pubmed-meshheading:3858829-Base Sequence,
pubmed-meshheading:3858829-DNA,
pubmed-meshheading:3858829-Female,
pubmed-meshheading:3858829-Genes,
pubmed-meshheading:3858829-HLA-DR Antigens,
pubmed-meshheading:3858829-Histocompatibility Antigens Class II,
pubmed-meshheading:3858829-Isoelectric Point,
pubmed-meshheading:3858829-Major Histocompatibility Complex,
pubmed-meshheading:3858829-Molecular Weight,
pubmed-meshheading:3858829-Oocytes,
pubmed-meshheading:3858829-Xenopus laevis
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pubmed:year |
1985
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pubmed:articleTitle |
DNA sequence and characterization of human class II major histocompatibility complex beta chains from the DR1 haplotype.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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